That elder flowers had moderately strong anti-inflammatory properties, however, no further research to back this claim has been carried out. Recent research carried out at Tufts University has revealed that elderberry contains four anthocyanins that work to protect endothelial cells, which line artery walls, from oxidative damage caused by free radicals. THERAPEUTIC DAILY AMOUNT: Elderberry comes in tinctures, liquid extracts, lozenges, syrups, standardized extract capsules, and throat sprays. Follow dosage directions on labels. MAXIMUM SAFE LEVEL: Not established SIDE EFFECTS CONTRAINDICATIONS: No adverse reactions to elderberry are known to exist. Raw berries are edible but may cause nausea and vomiting. Herbal products made from the leaves, stems or bark of the elderberry tree should NOT be taken internally as they contain the potentially fatal poison cyanide. Feverfew Tanacetum parthenium ; GENERAL DESCRIPTION: Feverfew was commonly used by the early Europeans and Greeks to treat fevers, headaches, arthritis, menstrual problems, and other generalised aches and pains. Today, the plant is most commonly used to reduce the frequency and severity of migraines - several placebo-controlled human trials have confirmed feverfew's effectiveness in this area. The active ingredient in feverfew is a compound called parthenolide, which belongs to a group of compounds known as sesquiterpene lactones. Parthenolide prevents the excessive clumping of platelets in addition to inhibiting the release of certain chemicals, including serotonin and some inflammatory mediators. Feverfew's anti-migraine action was originally attributed to its parthenolide content, however this has been a subject of recent debate. ROLE FOR ANTI-AGING: Research carried out by Mazor et al in 2000 suggests that sesquiterpene lactones inhibit the expression of the immunoreactive molecule Interleukin IL ; 8, a discovery that could account for feverfew's anti-inflammatory actions. One study also found that low concentrations of parthenolide were able to inhibit growth of tumor lines in vitro. THERAPEUTIC DAILY AMOUNT: Feverfew is sold dried and in capsules, concentrated drops, tinctures and extracts. The newest products are standardized for 0.1 to 0.2% of the chemical parthenolide however standardized leaf extracts may contain as much as 0.7%. An average daily dose is 125mg of feverfew standardized for 0.2% parthenolide ; or 250mcg parthenolide. A number of studies done in the last 10 years indicate that several commercial feverfew products contained none of the active compound parthenolide in addition, parthenolide levels of the dried herb were found to fall during storage ; . These studies emphasize the importance of using high-quality standardized extracts of this herb in order to obtain proper dosage and reliable effects. MAXIMUM SAFE LEVEL: Not established SIDE EFFECTS CONTRAINDICATIONS: Few side effects have been associated with.
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1. 2. 3. McMullin M, Johnston G. Long term management of patients after splenectomy. BMJ 1993; 307: 1372-3. Mayon-White R. Protection for the asplenic patient. Prescriber's Journal 1994; 34: 165-70. Amman AJ, Addiego J, Wara DW, Lub B, Smith WB, Mentzer WC. Polyvalent pneumococcal polysaccharide immunisation of patients with sickle-cell anaemia and patients with splenectomy. N Engl J Med 1977; 297: 897-900. Read RC, Finch RG. Prophylaxis after splenectomy. JeAntimicrob Chemother 1994; 33: 4-6. Immunization Practices Advisory Committee. Update: pneumococcal polysaccharide vaccine usage United States. MMWR 1984; 33: 273-81. Department of Health, Welsh Office, Scottish Home and Health Department, Department of Health and Social Services Northern Ireland. Immunisation against infectious disease. London: HMSO, 1992: 100-3. Department of Health. Asplenic patients and immunisation. CMO's Update 1994; 1: 3. Jones DM, Kaczmarski EB. Meningococcal infections in England and Wales: 1994. Communicable Disease Report 1995; 5: R125-30. Baddeley PG. Splenectomy and prevention of overwhelming infection. Hospital Update 1993; 19: 365-7. Department of Health. Hospital episode statistics, England: financial year 1993-4 . Vol 1. London: Government Statistical Service, 1995. Kinnersley P, Wilkinson CE, Srinivasan J. Pneumococcal vaccination after splenectomy: survey of hospital and primary care records. BMJ 1993; 307: 1398-9. Kristensen K. Antibody response to a Haemophilus influenzae type b polysaccharide tetanus toxoid.
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1. Department of Medicine for the Elderly, Leeds General Infirmary 2. Department of Health Sciences, University of York 3. Department of Medicine, York Hospital!
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The existence of FM was questioned and the meeting heard how difficult it is to work out the biology of FM. On the question of whether FM actually exists it was reported that at a recent neuro surgeon conference many thought it did not exist. Comment was made that if a drug comes on the market for FM it is question of getting medics to use it. There is a major impact in USA and Germany because professional people are driving the research. In the UK we only have FMA UK. There is a need for medics who believe to drive it here. In the UK medics wait to see what happens in the USA before using a drug here. There is a need for professional processes for FM. Reference was made to the 1992 criteria WHO Copenhagen Convention on FM ; , which included more criteria than just tender points. Comment was made that in the USA medics are more thorough because they have more time. It is hoped the European countries will be looking at reassessing the diagnosis and vicoprofen.
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8-oxo-dG was performed using an ESA Coulochem dual coulometric detector model 5200; ESA, Bedford, MA ; equipped with a 5011 analytical cell. Assays. Standard enzymatic methods were used to measure levels of creatine kinase CK ; , aspartate aminotransferase AST ; , and lactate dehydrogenase LDH ; in mouse serum 20 ; . Measurement of glutathione. GSH was measured spectrophotometrically using glutathione-S-transferase 21 ; . GSSG was assayed by an HPLC method with detection at 365 nm, which we recently developed to measure GSSG in the presence of a large excess of GSH 22, 23 ; . Measurement of MDA. MDA content was determined in skeletal muscle mitochondria using an HPLC method described by Wong et al. 24 ; that determines MDA formed from lipoperoxides. mt lipoperoxides are hydrolyzed by boiling in diluted phosphoric acid. MDA reacts with thiobarbituric acid to form an adduct, which is detected by HPLC. The calibration curve was assayed using tetramethoxypropane, which undergoes hydrolysis to liberate stoichiometric amounts of MDA 25 ; . Measurement of peroxide production by mitochondria. After isolation, skeletal muscle mitochondria were suspended in ice-cold respiratory buffer with 0.3 M sucrose, 1 mM EGTA, 5 mM Mops, 0.1% BSA, and 5 mM KH2PO4 adjusted to pH 7.4 with KOH ; and were used to measure the production of peroxides with an Epics Elite II flow cytometer Coulter Electronics, Hialeah, FL ; . Dihydrorhodamine 123 was used as fluorochrome on incubation at 37 C for 30 min and was excited with an argon laser tuned at 488 nm. Colorless dihydrorhodamine 123 is oxidized outside mitochondria by H2O2-dependent reactions to the fluorescent product rhodamine 123, which stains mitochondria 26, 27 ; . Rhodamine 123 is a cationic fluorescent lipophilic dye with a delocalized positive charge. It is readily taken up by active mitochondria without inducing cytotoxic effects. The mt membrane potential is the driving force for rhodamine 123 uptake by mitochondria 26 ; . This reduced rhodamine has been used to monitor reactive oxygen intermediates in different cellular and subcellular models 27, 28 ; . The gate was set on logarithm of forward and side scatter to always include mitochondria with the same morphological properties. Thus, changes in fluorescence were independent of the particle size. Electron microscopy of mouse muscle samples. Skeletal muscle samples gastrocnemius ; were obtained for electron microscopy. Samples were fixed in 0.1 mM phosphate buffer pH 7.2 ; containing 2.5% glutaraldehyde for 2 h, and then postfixed in 0.1 mM phosphate buffer pH 7.2 ; containing 2% osmium tetroxide for a further 2 h. After embedding the sample blocks in Epon, ultrathin sections were cut with the ultramicrotome Ultratome III Ultratome, Uppsala, Sweden ; and then contrasted with uranyl acetate and lead citrate for transmission electron microscopy. Electron microphotographs were taken with an electron microscope JEOL, Tokyo, Japan ; JEM 11 B. Statistics. Results are expressed as the mean SD. Statistical analyses were performed by the least significant difference test, which consists of two steps. First an ANOVA was performed. The null hy.
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