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Elite athletes are supposed to have tough, strong bodies, but several studies show that former elite athletes have a much higher incidence of arthritis than non-athletes Medical Journal of Australia , February 1997 ; . Many factors cause arthritis: an overactive immune system, as in rheumatoid arthritis, when your antibodies and cells attack and damage your joints; an infection in your joint; crystals forming in and grinding away your cartilage, as in gout; or a gradual wearing away of cartilage called osteoarthritis. Osteoarthritis is the type that is common among former elite athletes. That may make you think that it's not a good idea to compete in sports. However, the data show that casual exercisers are far less likely than non-exercisers to suffer osteoarthritis. The most likely explanation for the higher incidence of arthritis in former elite athletes is that the intense competitive drive that it takes to be a champion causes them to endure joint injuries and damage cartilage. They may injure their joints during competition, or they ignore pain and train through wear and tear injuries and suffer joint injuries that never heal. What does this mean to the casual exerciser? Make sure that you set up a program in which you do not exercise intensely on consecutive days and stop when you feel pain. It's exercising when you hurt that leads to osteoarthritis in elite athletes.
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FIG. 3. Effect of ligands on the exchange of AR at its cognate binding site. A to E ; FRAP analysis of GFP-AR at the MMTV array. The 3108 cells were treated with R1881 10 8 M ; for 45 min. The cells were imaged before and during recovery after bleaching of GFP-AR. Images collected at 10 s and 60 s E ; demonstrate the rapid recovery kinetics of GFP-AR at the MMTV array. Bar in panel E, 4 m. F ; Quantitative FRAP analysis of GFP-AR at the MMTV array. Cells were either left untreated or were treated with 10 8 M R1881, 10 8 M DHT, 10 8 M TST, or 10 6 M RU486 for 45 min and with 10 6 M CPA, 10 6 M bicalutamide Casodxe [CAS] ; , or 10 6 OHF for 90 min. For each set of conditions, at least 25 cells from three independent experiments were analyzed. G ; Time for 50% recovery of fluorescence t1 2 ; for GFP-AR on the MMTV array in the presence of the various ligands. The t1 2 values were calculated from the recovery curves n 25 for each ligand condition error bars represent standard errors.
3.3. Casodex-induced limited DNA Fragmentation in LNCaP cells Formation of nucleosomal DNA ladders is one of the characteristic features of apoptotic cell death. TNF- induces apoptosis in LNCaP cells and is well known to induce the formation of oligonucleosomal ladders. As shown in Fig. 2.
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